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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Applied Photophysics multiple sequence alignment embl ebi
Fig. 1. Nucleotide and deduced amino acid sequences of QsMT <t>(EMBL</t> accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.
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Image Search Results


Fig. 1. Nucleotide and deduced amino acid sequences of QsMT (EMBL accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.

Journal: Journal of experimental botany

Article Title: A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress.

doi: 10.1093/jxb/erh254

Figure Lengend Snippet: Fig. 1. Nucleotide and deduced amino acid sequences of QsMT (EMBL accession number AJ577299). (A) The ORF (indicated in uppercase) encodes a putative protein of 77 amino acids including 14 cysteines distributed in two Cys-rich regions. Two introns interrupt the coding region at the points signalled by arrowheads. Oligonucleotides used for QsMT gene amplification are boxed. (B) DNA sequence of QsMT introns 1 and 2 (see arrowheads in A). A region of approximately 500 bp of the intron 1 (marked by parallel lines) remains unsequenced.

Article Snippet: Clone sequences were identified using an EMBL DataBank BLAST search.

Techniques: Sequencing

Fig. 2. Protein distance tree constructed with the plant MT sequences retrieved by a BLAST search in the EMBL database, using QsMT as a query and an E-value cutoff of 0.028. Alignment was performed using Clustal W and the phylogenetic tree was constructed using the MEGA software, version 2.1 (Kumar et al., 2001). Nearly all sequences retrieved were type 2 MTs, except for a small cluster of type 1 MTs (bordered). QsMT is indicated by an arrow.

Journal: Journal of experimental botany

Article Title: A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress.

doi: 10.1093/jxb/erh254

Figure Lengend Snippet: Fig. 2. Protein distance tree constructed with the plant MT sequences retrieved by a BLAST search in the EMBL database, using QsMT as a query and an E-value cutoff of 0.028. Alignment was performed using Clustal W and the phylogenetic tree was constructed using the MEGA software, version 2.1 (Kumar et al., 2001). Nearly all sequences retrieved were type 2 MTs, except for a small cluster of type 1 MTs (bordered). QsMT is indicated by an arrow.

Article Snippet: Clone sequences were identified using an EMBL DataBank BLAST search.

Techniques: Construct, Software